Novel g.2055A>C and g.2064T>A Polymorphisms of KISS1 Gene and Its Association with Reproductive Traits in Local Indonesian Goats

Kisspeptins are known as neuropeptides encoded by the KISS1 gene, which potentiates GnRH neuron excitability. Unfortunately, role of gene in reproductive traits remains unclear Indonesian native goat breeds. The current study purposed to detect genetic variation and investigate association with goats acquire marker-assisted selection (MAS) for breeding program. Further, ninety blood samples from randomly selected animals were used DNA isolation SNP genotyping. serum was collected sixteen treated does assess FSH levels using ELISA method. differences between studied parameters determined independent T-test SAS Software. Two SNPs, g.2055A>C (SNP1) g.2064T>A (SNP2) discovered intron 1 genotypes identified each SNPs: AA AC at SNP1 TT TA SNP2. allele A (0.96) T (0.93) SNP2 dominant alleles population. Furthermore, chi-square test showed that both SNPs under Hardy-Weinberg equilibrium. variants have no effect on litter size (p>0.05). Moreover, genotype had a higher level than observed (p<0.0001), especially follicular phase. not correlate relatively (p=0.23). MAS program could escalate